Experimental study on the therapeutic effect and underlining mechanisms of positron in pancreatic cancer cells

نویسندگان

  • Ying Wang
  • Ming Li
  • Rao Diao
  • Brian Tung
  • Dalong Zhang
  • Yaming Li
چکیده

The purpose of this study was to assess the potential therapeutic effect of positrons emitted by 18F-2-Deoxy-2-Fluoro-D-Glucose (18F-FDG) on pancreatic cancer cells and elucidate its underlying mechanisms. Pancreatic cancer cells were incubated with different radioactive concentrations of 18F-FDG and evaluated for anti-cancer properties and underlining mechanisms. In addition, three groups of tumor-bearing mice were treated with different doses of 18F-FDG weekly, the tumor growth rate was calculated, and the mice were imaged by positron emission tomography (PET) with 18F-FDG before and after treatment. The presence of apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) stain and immunohistochemistry analysis. All treated groups exhibited positron-inhibited proliferation and positron-induced apoptosis compared with the control group in vitro. Further, we noted that higher treatment dose correlated with a better treatment response. In vivo, the high dose administration of 18F-FDG reduced tumor growth and prolonged the survival of treated mice compared with the control group with no change in the behavior or normal tissues of the mice. Immunohistochemical analysis and TUNEL stain showed more apoptotic cells than that in control group. The results demonstrated that positron radiation inhibited the proliferation and induced apoptosis of pancreatic cancer cells in vitro and in vivo, via an endogenous mitochondria-mediated signaling pathway.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Analysis of epithelial mesenchymal transition markers in breast cancer cells in response to stromal cell-derived factor 1

Introduction: Metastasis is the main cause of cancer death; however, the underlying mechanisms of metastasis are largely unknown. The chemokine of stromal cell-derived factor 1 (SDF1) and the process of epithelial mesenchymal transition (EMT), both have been declared as important factors to promote cancer metastasis; however, Conspicuously, the relation between them has not been recognized well...

متن کامل

Cytotoxic effect of pyocyanin on human pancreatic cancer cell line (Panc-1)

Objective(s): Pyocyanin, a blue-green pigment produced by Pseudomonas aeruginosa, interferes with host redox cycles, which can lead to generation of reactive oxygen species and progressive cellular oxidative damage. The aim of this study was to assess the influence of pyocyanin on human pancreatic cancer cell line.Materials and Methods: Polymerase Chain Reaction (PCR) was applied to confirm the...

متن کامل

Effects of Lycium barbarum. polysaccharide on type 2 diabetes mellitus rats by regulating biological rhythms

Objective(s): Type 2 diabetes mellitus (T2DM) is associated with circadian disruption. Our previous experimental results have showed that dietary Lycium barbarum. polysaccharide (LBP-4a) exhibited hypoglycemic and improving insulin resistance (IR) activities. This study was to explore the mechanisms of LBP-4a for improving hyperglycemia and IR by regulating biological rhythms in T2DM rats. Mat...

متن کامل

The Effect of Eight Weeks of Aerobic Exercise on the Expression of Senescence Proteins P53 and P16 in Pancreatic Tissue of Diabetic Mice

Background: Chronic hyperglycemia is associated with an increase in cellular damage due to oxidative stress and increases insulin resistance and also increases in p53 and p16 beta cells, leading to the induction of senescence in pancreatic insulin-secreting cells. The aim of this study was the effect of eight weeks of aerobic exercise on the expression of senescence proteins P53 and P16 in the ...

متن کامل

The Role of Microgravity in Cancer: A Dual-edge Sword

Since human beings could travel beyond the earth atmosphere, scientists started to investigate the effect of microgravity on human cells. Microgravity has different effects on normal and cancer cells, but the related mechanisms are not well-known till now. The aim of the present review is to focus on the consequences of exposing the cancer cells to reduced gravity. Some cancer cells organize th...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 8  شماره 

صفحات  -

تاریخ انتشار 2017